Molecular Detection Of Mycobacterium Bovis In Cattle Milk And Abattoir Staff In Enugu

ABSTRACT The study was carried out to detect Mycobacterium bovis in cattle milk and abattoir staff in Enugu. Blood samples were collected from 50 subjects at Artisan and Ogbete market abattoirs with 25 samples from each location. Fifty Milk samples were collected from cattle at Fulani settlements which comprised of 13 from Gariki, 16 from 9th Mile and 21 from Monarch in Enugu. Milk samples were cultured on Lowenstein Jensen medium and incubated for 8weeks at 37o C. DNA was extracted from both milk and blood samples using Relia prep DNA spin column method and screened for Tuberculosis using Ne…