Evaluation Of The Antinociceptive And Anxiolytic-like Activities Of Leaf Extract And Fraction Of Phyllanthus Muellerianus Kuntze

ABSTRACT The anxiolytic and antinociceptive properties of the leaf extract and fraction of Phyllanthus muellerianus Kuntze (Phyllanthaceae) were investigated.The extract (MME; 500.18 g; 10%w/w), obtained by cold maceration in 1:1 mixture of methylene chloride: methanol for 48 h, was fractionationed in a silica gel column which yielded the ethyl acetate fraction (EF; 199.2 g; 39.83% w/w). The extract and fraction were subjected to phytochemical analysis. Acute toxicity of MME was determined in mice using the oral route. Anxiolytic-like activity of MME and EF was investigated in mice using behav…